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Dr Paulo Jorge Silva Oliveira: “Mitochondrial Remodeling during Embryonal Cancer Stem Cell Differentiation”

15th July 2015 @ 3:00 pm - 4:00 pm

Free
Wed, 15 July, 2015
3:00 pm
Lecture Theatre, Level 7
Host: Michael Murphy
Dr. Paulo Jorge Silva Oliveira
University of Coimbra, Portugal

The relationship between mitochondrial metabolism and cell viability and differentiation in stem cells (SCs) remains poorly understood. We compared mitochondrial physiology and metabolism between embryonal carcinoma P19-SCs before/after retinoic acid differentiation and observed an unique fingerprint of the association between mitochondrial activity, cell differentiation and stemness. In comparison with their differentiated counterparts, pluripotency of P19-SCs was correlated with a strong glycolytic profile and decreased mitochondrial biogenesis and complexity: round, low-polarized and inactive mitochondria with a closed permeability transition pore. This decreased mitochondrial capacity increased their resistance against dichloroacetate. Thus, stimulation of mitochondrial function by growing P19-SCs in glutamine/pyruvate-containing medium reduced their glycolytic phenotype, induced loss of pluripotent potential, compromised differentiation and became P19-SCs sensitive to dichloroacetate. We also demonstrated that melatonin cytotoxic effects depend on mitochondrial function. Due to the central role of this type of SCs in teratocarcinoma development, our findings highlight the importance of mitochondrial metabolism in stemness, proliferation, differentiation, and chemoresistance.

Details

Date:
15th July 2015
Time:
3:00 pm - 4:00 pm
Cost:
Free
Event Category:
Website:
http://www.mrc-mbu.cam.ac.uk/seminars

Organiser

MRC Mitochondrial Biology Unit
View Organiser Website

Venue

Lecture Theatre, Wellcome Trust/MRC Building Level 7
Addenbrooke’s Hospital
Cambridge, CB2 0XY
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