The Masterclass is a series of lecture/discussion forum presented by postdocs that provides unique opportunities for researchers/students in the biological sciences to:

• Share and discuss current hot topics in research fields.

• Present and discuss new cutting edge techniques, skills and tools being used in laboratories.

• Get together and discuss possible collaborations between and across laboratories.

• Learn about less familiar subject areas.

• Network and make friends across laboratories and departments.

• Promote university facilities and resources.

• With free pizza, beer, and wine

All (students, P.I.s, technicians etc.) are welcome!

Fluorescence recovery after photobleaching (FRAP) – from data acquisition to results interpretation

Fluorescence recovery after photobleaching (FRAP) is widely used to study dynamics of proteins and protein complexes in living cells. In this Masterclass, I will start by giving an overview of the FRAP technique. Then, I will discuss the technical considerations involved in FRAP experiments, common approaches to interpret the results, and available tests to validate the interpretations. Finally, I will demonstrate examples of how FRAP was used to investigate protein dynamics in a range of biological systems.

• Natalia Bulgakova (Gurdon Institute)
• Wednesday 18 March 2015, 18:30–20:00
• Gurdon Institute Tea Room.