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Seahorse Workshop

9th May 2016 @ 9:30 am - 3:30 pm

Free
Attendees are invited for this event Measuring Metabolic Engines & Fuels with the Seahorse XF Analyzer
 
Metabolism is the key to understanding cell function. In living cells, most of the energy produced is derived from three fuel sources: glucose, glutamine, and fatty acids. Mitochondrial access to these fuels impacts a wide variety of biological processes.
Please see attached flyer
 
Please register by May 4, 2016 and indicate if you would like to join the wet lab session
email Karen Hébert kah33@mrc-cu.cam.ac.uk

Using the Seahorse XF Analyzer you can:

  • Determine whether/how cells can adjust fuel oxidation to match nutrient availability while meeting energy demand.
  • Distinguish metabolic adaptations due to genetic changes vs. those that take place due to nutrient deprivation.
  • Identify fuel dependencies to uncover cancer
    cell vulnerabilities.
  • Explore how fuel preferences lead to cell fate decisions for differentiation and immune cell activation.

 

Agenda:

Monday, May 9, 2016

9:15 – 9:30  Participant Introduction

9:30 – 10:15   Seahorse Intro Presentation   – Sofia Vikstrom

10:15 – 11:00  Software & Assay Optimization   – Sofia Vikstrom

11:00 – 11:15  Coffee Break

11:15 – 12:00  “Phenotypic characterisation of   mtDNA mutation heteroplasmy shift”   – Edoardo Gaude

12:00 – 13:00  Lunch

13.00 – 13.30  “Circadian Biology”   – Priya Crosby

13:30 – 14:00  “Using the Seahorse to explore mitochondrial function”   – Hiran Pirag

14:00 -14:30   “Hypoxia Biology”   – Stephen Burr

14:30 – 15:00   “Mitochondrial Kidney Disease”   – Simon Hoer

15.00 – 15:30  Wet Lab Part I (Limited attendees)

Tuesday, May 10, 2016

9:00 – 14:00  Wet-Lab Part II (limited attendees!)

Details

Date:
9th May 2016
Time:
9:30 am - 3:30 pm
Cost:
Free
Event Category:

Venue

Room 1.8 Hutchison/MRC Research Centre
Cambridge, CB2 0XZ United Kingdom + Google Map