A Simple High-Throughput Method for the Comprehensive Extraction of Lipids
Protocol category- LC-MS methods
Your nameDr Ben Jenkins
Your organisationIMS-MRL, Cambridge
Key wordsodd chain lipids; lipid profiling; Folch; protein precipitation; sample preparation; relative lipid composition
Typical lipidomics methods incorporate a liquid–liquid extraction with LC–MS quantitation;
however, the classic sample extraction methods are not high-throughput and do not perform well
at extracting the full range of lipids especially, the relatively polar species (e.g., acyl-carnitines and
glycosphingolipids). In this manuscript, wepresent a novel sample extraction protocol, which produces
a single phase supernatant suitable for high-throughput applications that oers greater performance
in extracting lipids across the full spectrum of species. We applied this lipidomics pipeline to a
ruminant fat dose–response study to initially compare and validate the dierent extraction protocols
but also to investigate complex lipid biomarkers of ruminant fat intake (adjoining onto simple odd
chain fatty acid correlations). We have found 100 lipids species with a strong correlation with
ruminant fat intake. This novel sample extraction along with the LC–MS pipeline have shown to
be sensitive, robust and hugely informative (>450 lipids species semi-quantified): with a sample
preparation throughput of over 100 tissue samples per day and an estimated ~1000 biological fluid
samples per day. Thus, this work facilitating both the epidemiological involvement of ruminant
fat, research into odd chain lipids and also streamlining the field of lipidomics (both by sample
preparation methods and data presentation).
Measuring COQ
Protocol category- LC-MS methods
Your nameProf. Mike Murphy
Your organisationMRC MBU
Key wordsCo Q
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Waffle about CoQ
Protocol category- targeted analyses
Your nameMr freed smith
Your organisationMs
Key words1 2 3
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Protocol category- untargeted analyses
Your nameRev. John Smith
Your organisationChurch
Key wordsnone
Teaxt and file
Protocol category- GC-MS methods
Your nameMs Jane Sugars
Your organisationMRL
Key wordsHello
testing, testing
Long piece of text
Protocol category- Metabolite extraction
Your nameMs Jane Sugars
Your organisationMRL
Key wordsnone
none 2
Protocol category- GC-MS methods
Your nameMs Jane Sugars
Your organisationMRL
Key wordsnone
None
Protocol category- GC-MS methods
Your nameMs Jane Sugars
Your organisationMRL2
Key wordsTest, test 3
Test 3 text
Test title 2
Protocol category- untargeted analyses
Your nameMs Jane Sugars
Your organisationMRL
Key wordsTest 2
Test text 2
Test title one
Protocol category- GC-MS methods
Your nameMs Jane Sugars
Your organisationMRL
Key wordsTest
Test protocol text